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Astigmatic traction force microscopy (aTFM), Nat Commun, 12 Apr 2021

发布时间:2021年04月12日

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Nature Communications, 12 April, 2021, DOI:糖果派对网站网址5771

Astigmatic traction force microscopy (aTFM)

Di Li, Huw Colin-York, Liliana Barbieri, Yousef Javanmardi, Yuting Guo, Kseniya Korobchevskaya, Emad Moeendarbary, Dong Li & Marco Fritzsche

Abstract

Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity.

文章链接:http://www.9797SB.COM/374/articles/s41467-021-22376-w

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